Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Do NOT use the front panel Ref button in fluorescence mode cuvette reads. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. . 4. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Bottom-reading is usually recommended for cell-based assay with adherent cells. The information below applies to a standard 96 well plate, set to a linear shake mode. The resorufin product formed was monitored by fluorescence spectroscopy (excitation energy at 530 nm and emission energy at 590 nm) every 10 min in Spectramax M3 (Molecular Devices). 312 gain calibration failed. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 标准功. 306 plate air cal. Learn how to set up protocols, customize reports, export results, and more with this PDF document. spr SpectraMax iD5 SpectraTest ABS2 v1. Description 1. Ataxia telangiectasia and Rad3-related (ATR) kinase protects genome integrity during DNA replication. Molecular Devices SpectraMax M3 User Manual Download Operation & user’s manual of Molecular Devices SpectraMax M3 Laboratory Equipment for Free or View it Online on All-Guides. The Molecular Devices® SpectraMax® M3 Reader provides three detection modes: UV-Vis absorbance, fluorescence intensity, and luminescence, but can be upgraded to include time-resolved fluorescence and/or fluorescence polarization modes. 304 signal level saturation. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. book Page 1 Tuesday,. SpectraMax readers are the only ones on the market with the versatility to evolve and adapt to every need. Microplate Readers Molecular Devices VersaMax Microplate Reader. FlexStation™ II. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. StakMax Microplate Handling Stacker. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The resulting absorbance values were plotted against time. Other technologies such as imaging. Offer Ends In: 5 Days 05: 02: 03. Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. 0200-6117. Carefully place the cytometer on its side on the foam pad so that the top of the cytometer faces the bottom of the microplate reader. AquaMax® 2000. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence. 1step assay with SpectraMax microplate readers. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. SpectraMax® ABS Plus. The exactly same condition without CCK-8 solution was used to subtract background signal that is absorbed by cell medium or natural product. The chaperone function of PfHsp70-1 under various experimental conditions. User Manual – SpectraMax M3 M4 M5 M5e. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. All procedures completely complied with the manufacturer’s instructions. 4. 1). The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 1 & 7. The cuvette must be in place when you read the plate. D 1 1. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). Description 1. Examples: “Press carefully to engage,” “ Do not press down. The assay was repeated at varying final concentrations (5, 15, 25, 50, 100 nM) of PES. Polyplex Size and Zeta Potential Measurement. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. SpectraMax Luminometer Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Reference and the Water Constant methods. Features. Our Price: $8,635. Figure 2-23: Bottle holder To install the bottle holder: 1. Note: In this user guide, all references to SpectraMax Multi-Mode. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeNote: In this user guide, SpectraMax ® refers to several SpectraMax . SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 72 MB. The reading was taken with a spectrophotometer (SpectraMax M3) at 640 nm. The SpectraTest ABS1 Validation Plate is a comprehensive optical validation package that is com-pletely automated when used with SoftMax ® Pro software. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). SpectraMax Microplate Readers Multi-mode Readers Single-mode readers SoftMax Pro Software GxP Compliance Solutions SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. StakMax. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax® Microplate Readers and SoftMax® Pro Software. 0nm increments. Also refer to this Multimode Reader Comparison Chart. Extramitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer SpectraMax® M3 at 540 nm emission and 580 nm. Fundamentally, one is interested in whether or not an instrument can detect a signal for a given assay; that is, whether a signal is detectable above the background of the instrument. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. A single colony of bacterial isolates was inoculated in Marine Broth using a sterile inoculation loop. 9000-0756. The following document is intended to demonstrate setup of this instrument. spr SpectraMax iD3 SpectraTest ABS2 v1. Figure 6: Transcreener ADP2 FI assay principle. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Louis, USA). A purple product is formed when pLDH is present , and this product is quantified in a Spectramax M3 microplate reader at the absorbance of 620 nm. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. ” Download for Windows of SoftMax® Touch Software. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral. 5. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 7°C and the bottom to 36. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Cables. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. VersaMax ELISA. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The optical densities (OD) were determined using the SpectraMax ® Plus 384 Microplate Reader (SpectraMax M3; Molecular Devices, SanJosé, CA, USA) at 450 nm and 660 nm [31,34]. Published Dec 16, 2020 | Updated Dec 16, 2020. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. spr SpectraMax iD5 SpectraTest ABS2 v1. 10. Learn more about its features, specifications, and applications in this user. 7 mm for all shake intensities. Multimode Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. 3 or greater. Sale. 1. When a feature or capability applies to only certain readers, this exception is noted. Wafi’s main research topic aims to set up a pharmacodynamic model comparing antibiotic activities against biofilms and examine in parallel their effects on viability and biofilm mass. 8. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode. 313 reference gain check fail. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. Multimode Reader Comparison Chart. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Page 22 StakMax Microplate Handling System User Guide 3. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. For more sample throughput, the system canStakMax Handling Stacker Base Unit. Fluorescence Microplate Reader Comparison Chart. Absorbance was measured at 490 nm using a microtiter plate reader (Molecular Devices Spectramax M3, Sunnyvale CA, USA). 1. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. I used the same plate and set the PMT to be automatic. Multimode Reader Comparison Chart. The drawing shows a standard microplate of 15 mm height covered with a lid. Supplier: Molecular Devices. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. SpectraMax® M5e. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. SpectraMax M3 Multi-Mode Microplate Reader. 7. The results were normalized as percentage based. SpectraMax M3, M4, and M5/M5e readers. The corrected absorbance is within 5% of the. Fluorescent Polarization: Solid black plates, as above. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. For wavelengths in the UV range above 220 nm,. SpectraMax M2, M2e;. book Page 21 Wednesday, July 7, 2010 2:09 PM. book Page 1 Wednesday, July 7, 2010 2:09 PM. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. All associated StakMax® stackers. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence,. SpectraMaxi3Multi. Acarbose was used as a. 314 low lamp level warning. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Transfection with siRNA. Metrics used to measure sensitivity include signal-to-background ratio (S/B), signal-to-noise ratio (S/N), lower limit of detection (LLD) and Z’-factor 1. Spectral Range. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Speed Read Mode. 9000-0756. Bioz Stars score: 86/100, based on 1 PubMed citations. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. Detection modalities are shown in Table 1-1. Page 22 SpectraMax L Luminescence Microplate Reader User Guide To manually change the aperture: 1. 4. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The positive control was treated with fresh RPMI-1640 (Lonza, Walkersville, MD). 2. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Description 1. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper Module. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9 SpectraMax® L (software version 5. Aggregation of MDH was monitored as a function of light scattering at 360 nm over 30 min at 51°C in a SpectraMax M3 (Molecular Devices, United States) microplate spectrometer. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. The team have compared the effects of antibiotics on bacterial viability and. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. These lab workhorses are used in drug discovery, research, bioassay validation, and. SpectraMax Paradigm Multi-Mode Safety Guide 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. If filters are used with the SpectraMax iD5 reader, integration time may be set to 50 ms, reducing the. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. The innovative and flexible design features. . Bioz Stars score: 86/100, based on 1 PubMed citations. Use the 3. LINKS to Craig…His YouTube is @BrickArmy and his business is: is COBI’s website: set in this video is #2535 M. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. RP-3500 is highly selective for ATR with 30-fold selectivity over mammalian target of rapamycin (mTOR) and more than 2,000-fold selectivity over ataxia. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 9000-0756. Bioz Stars score: 96/100, based on 1 PubMed citations. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. ATP standard curves read on SpectraMax microplate readers. Find regional support. Instructions for using SIRT1 siRNA and scrambled siRNA were provided by Santa Cruz Biotechnology (CA, USA). The absorbance was read using a UV–visible spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at a wavelength of 765 nm. Data were collected using SoftMax Pro 6. 303 unable to cal dark current. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 2. 3 software (Molecular Devices, San Jose, CA, USA). In the M2, M5 and Plus 384, the light. Hydrophobicity assayThese settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. 4. . Qualify the fluorescence performance of the SpectraMax . Resolution: The validation protocols are included in the v5. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SpectraMax® M5. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). 3: The cuvette chamber. Samples were excited at 485 nm, and fluorescence intensity was measured at 520 nm on a fluorometer (SpectraMax M3; Molecular Devices, Sunnyvale, CA). a SpectraMax M3 microplate reader (Molecular Devices Inc. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. Molecular Devices SpectraMax M3 User Manual (86 pages) Multi-Mode Microplate Readers. 0. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. To avoid injury, it is recommended that two. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 0 out of 5 $ 11,999. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. A BCIP/NBT ALP staining kit (Beyotime Institute of Biotechnology, Shanghai, China) was utilized to perform ALP staining on the 7th day according to the manufacturer's instructions. For assays where cells were pre-treated with ABAH or vehicle control, cells were incubated in. Bithionol (2 μg/ml) and vancomycin (4 μg/ml) were used as controls. . The detection of intracellular ROS and mitochondrial membrane potential. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Among the various ready-to-read protocols, users will find pre-written SpectraDrop Plate protocols enabling Micro Volume applications measurement. . While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 3. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Our absorbance plate readers feature our PathCheck Sensor. For a complete list of the instruments supported by this release of the8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Recommended SpectraMax iD3/iD5 reader settings. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 0 out of 5 $ 12,499. e. Twenty five μL of supernatant was removed from each sample and placed in a 96-well clear bottom plate and read at 546/575 nm (excitation/emission) using a SpectraMax M3 fluorescent microplate reader (Molecular Devices, Sunnyvale, CA). Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Choose cables that meet the requirement described in this appendix. Add to Cart. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. As for each measurement, I. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. FI assay. Wait 20 seconds. Antiretroviral activities. Multimode Reader Comparison Chart. 1) EarlyTox Glutathione Assay (6. We would like to show you a description here but the site won’t allow us. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. 4 kilograms). M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 37) was ordered from Sigma Aldrich (St. Multimode Readers Comparison Chart. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Activity I Want To Make Friends… And I Need Your Help. Type: Clear: Add to Cart. High sample correlation coefficients (R 2) make it easy to. Choose from a three- (M3), four- (M4), orThe decrease in absorbance at 340 nm was measured for 20 min with a SpectraMax M3 microplate reader. 4/5 Set up plate template / Define Plate Map 1. ISM Equipment Classification (Group 1, Class A) This equipment is. The SpectraTest ABS1 Validation Plate package. 과 같은 Parameter를 검사하여 평가할 수 있습니다. book Page 1 Wednesday, July 7, 2010 2:09 PM When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. 3 software. SoftMaxProSoftwareUserGuide 2 5014177G ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevices,LLC(“Molecular Devices”)equipment,software,reagents. Neutrophils were stimulated as described in tissue-culture multi-well plates. 2 software (Sunnyvale, CA, USA). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 4. The guide also covers the software features, the specifications, and the accessories of the systems. A colorimetric assay was performed using a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. com. 3: The cuvette chamber. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Select wells and then add or select a group or blank and assignThe SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. S. 2. We would like to show you a description here but the site won’t allow us. Mitosox works fine in 96 wells. 3) At the bottom of the strip, click on "Show. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. SpectraMax® Paradigm® Multi-Mode Detection Platform. 1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. % EPS by weight was calculated by standardization of each mean concentration of proteins, carbohydrates, and eDNA to μg/ml, then divided by total starting weight of pooled crude EPS from ten samples. 1. The integration time suggested is 5-10 sec. If no sound is heard during initialization, contact technical support. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. SpectraMax M3, M4, M5, and M5 e. Microplate Readers include the M3, M4,. . ® reader to the computer. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. Place the microplate on or remove it from the microplate drawer. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forIntroduction. reader in the visible range between 405–750 nm with NIST and NMI. 2. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax ABS Plus SpectraTest ABS2 v1. STAKMAX. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Fluorescence of the samples were read using a SpectraMax M3 instrument with an excitation set to 355 nm and emission set to 460 nm. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Cells were cultured for at least 10 days in sphere-forming medium. Superior flexibilityThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. Detection modalities are shown in Table 1-1. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . SpectraMax ABS Plus SpectraTest ABS2 v1. The plates were protected from light and incubated at room temperature for 8 min. In addition, they allow to increase their performance sequentially from models: M2 ‘ M2e;. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. l. First, 4T1 cells were. Description 1. Published Dec 16, 2020 | Updated Dec 16, 2020. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Note: In this user guide, SpectraMax ® refers to several SpectraMax . Incubate at 37ºC for 1–4 hours. Microplate Reader Shelf. Cell Degranulation. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. FlexStation™ III. Choose from a three- (M3), four- (M4), orFluorescence was measured using a SpectraMax M3 plate reader equipped with an excitation at 490 nm and an emission at 535 nm.